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RP2: Microscopy

Preparation of stained squashes of cells from plant root tips; setup and use of an optical microscope

Required PracticalAQAA-Level

๐Ÿ”ฌ Equipment Required

  • Light microscope
  • Root tips (e.g., garlic, onion)
  • Slides and cover slips
  • Aceto-orcein or toluidine blue stain
  • Hydrochloric acid
  • Watch glass
  • Forceps
  • Mounted needle
  • Filter paper
  • Bunsen burner

๐Ÿฆบ Safety Precautions

  • Wear safety goggles
  • HCl is corrosive - handle carefully
  • Stains can be toxic and stain skin
  • Take care with Bunsen burner
  • Handle glass carefully

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About RP2: Microscopy

RP2: Microscopy is a required practical for AQA A-Level Biology. This practical preparation of stained squashes of cells from plant root tips; setup and use of an optical microscope

Students are expected to understand the method, identify and control variables, use appropriate equipment, analyse results, draw graphs, identify sources of error, suggest improvements, and consider safety precautions.

Questions on required practicals frequently appear in AQA A-Level Biology exams and can be worth significant marks. Practice with our AI-generated questions to master this practical.

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